Research Focus: Enzyme Engineering & Biocatalysis
Directed Evolution
Structure-based rational design
Structure-based rational design
Iterative mutagenesis and screening protocols to evolve enzymes with enhanced catalytic properties and stability profiles
Structure-based rational design
Structure-based rational design
Structure-based rational design
Structure-based rational design using molecular dynamics simulations and docking studies to predict beneficial mutations
High-Throughput Screening
Structure-based rational design
High-Throughput Screening
Rapid characterization platforms to evaluate thousands of enzyme variants for industrial applicability
Core Technical Capabilities
Purification & Characterization
Multi-step protein purification using FPLC/HPLC systems with rigorous kinetic profiling (Kmax, Vmax). Comprehensive substrate specificity analysis and structural stability assessment across varying pH and temperature ranges.
Immobilization Strategies
Development of robust techniques including covalent bonding, encapsulation, and cross-linking to enhance enzyme reusability and operational stability for continuous flow biotransformations in large-scale reactors.
Industrial Scale-Up
Translation of laboratory discoveries to pilot and production scales, ensuring consistent enzyme performance under real-world manufacturing conditions with cost-effective downstream processing.